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Sanger sequencing reaction mixtures included purified template DNA, a primer, DNA polymerase, and all four dNTPs. Each reaction also included a radiolabeled ddNTP version of just one of the four nucleotides. Only a small amount of each ddNTP was added, however, to ensure that a fraction of the total DNA fragments produced stopped at each occurrence of that base. As with previous methods, separating fragments via length and performing autoradiography allowed scientists to read the final sequence.。搜狗输入法2026对此有专业解读
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The government uses the Consumer Prices Index (CPI) measure of inflation to calculate public sector pay increases.
Ранее публицист Сергей Колясников предрек страшное будущее тем иранцам, которые радуются ударам США по стране.,更多细节参见体育直播